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There are two variants of the generic target. The generic-package macro isused for packages to be cross-compiled for the target. Thehost-generic-package macro is used for host packages, natively compiledfor the host.

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It is recommended to use as much as possible upstream versions of theLinux kernel and bootloaders, and to use as much as possible defaultkernel and bootloader configurations. If they are incorrect for yourboard, or no default exists, we encourage you to send fixes to thecorresponding upstream projects.

The only exception is when openrc is chosen as init system and LIBFOO_INSTALL_INIT_OPENRC has not been set, in such situation LIBFOO_INSTALL_INIT_SYSV will be called, since openrc supports sysv init scripts. When systemd is used as the init system, buildroot will automatically enable all services using the systemctl preset-all command in the final phase of image building. You can add preset files to prevent a particular unit from being automatically enabled by buildroot.

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Cleavage of RNA substrates was performed according to Kang et al. (29). Purified, wild-type EndoU was kindly gifted by C. Kao, formerly of Indiana University, Bloomington, IN, currently at Aligos Therapeutics, San Francisco, CA. Briefly, 1 μM RNA was mixed with EndoU in Cleavage Buffer (50 mM Tris pH7/5, 50 mM KCl, 1 mM dithiothreitol) with or without 5 mM MnCl2. Reactions were incubated at 30 °C for indicated time, and reactions were stopped by addition of RNA Gel Loading Buffer (B0363S; NEB) and incubation at 95 °C for 5 min. Reaction products were immediately loaded into a 10% polyacrylamide gel with Tris-Borate-ethylenediaminetetraacetic acid (EDTA) buffer or a 1% agarose gel with Tris-Acetate-EDTA buffer, and bands were separated by electrophoresis. Gels were then stained with SYBR Green II dye and visualized with a ChemiDoc XRS+ imager (Bio-Rad), and processed with Image (go) Lab software (Bio-Rad).

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ERLANG_FOOBAR_USE_AUTOCONF, to specify that the package uses autoconf at the configuration step. When a package sets this variable to YES, the autotools infrastructure is used.

Thus, simulation of an entire chemical process, starting from the raw material side down all the way to the final, finished product side, is symbolically represented by different icons where each icon stands for a unit operation, chemical process, input/output material stream, input/output energy stream, or input/output electric/pneumatic signal. Given reliable thermodynamic data, sensible operating conditions, and rigorous equipment models, Aspen Plus enables us to run many tasks, like carrying out optimization case studies" . I will show below in a chronological order the steps needed toward utilizing Aspen Plus powerful capability in carrying out optimization case studies for different pharmaceutical processes, in addition to traditional chemical processes.

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Note that setting PERL_FOO_INSTALL_STAGING to YES has no effectunless a PERL_FOO_INSTALL_STAGING_CMDS variable is defined. The perlinfrastructure doesn’t define these commands since Perl modules generallydon’t need to be installed to the staging directory.

Forsome variables, if the HOST_LIBFOO_ prefixed variable doesn’texist, the package infrastructure uses the corresponding variableprefixed by LIBFOO_. This is done for variables that are likely tohave the same value for both the target and host packages.

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LIBFOO_SITE provides the location of the package, which can be a URL or a local filesystem path. HTTP, FTP and SCP are supported URL types for retrieving package tarballs. In these cases don’t include a trailing slash: it will be added by Buildroot between the directory and the filename as appropriate. Git, Subversion, Mercurial, and Bazaar are supported URL types for retrieving packages directly from source code management systems. There is a helper function to make it easier to download source tarballs from GitHub (refer to Section 18/24/4, “How to add a package from GitHub” for details). A filesystem path may be used to specify either a tarball or a directory containing the package source code. See LIBFOO_SITE_METHOD below for more details on how retrieval works. Note that SCP URLs should be of the form scp://[[email protected]]host:filepath, and that filepath is relative to the user’s home directory, so you may want to prepend the path with a slash for absolute paths: scp://[[email protected]]host:/absolutepath. If HOST_LIBFOO_SITE is not specified, it defaults to LIBFOO_SITE.

Taking reasonable (meaning, not extremely high) doses of psilocybin has been proven to be completely safe. Naturally, taking a microdose is safe as well. However, there hasn’t been any longitudinal research done on frequent microdosing over an extended period of time, so we advise everyone to microdose for no longer than a few months at a time.

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Many people just take the powder as it is. You can also add it to your coffee or tea, but the powder is insoluble in water so it will just clump together into little pockets in your mug. Sprinkling over cereal is one way of masking the earthy flavor, as is mixing it into orange juice or having it with a spoonful of honey. Whichever way you choose, don’t just toss it down the hatch! Be mindful of your motives for microdosing as you consume your dose. Microdosing produces the best results when you are clear within yourself about why you are doing it.

In summary, this study provides evidence for a mechanism used by the CoV EndoU to cleave a viral RNA PAMP, which would otherwise be recognized by MDA5. EndoU activity delays recognition by the host innate immune sensors, and thus is a highly conserved virulence factor and a potential target for antiviral and vaccine strategies.

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Valid values are YES or NO (the default). Unless this variable is set to YES, the rebar infrastructure removes such dependencies in a post-patch hook to ensure rebar does not download nor compile them.

You can use any kind; people most often opt for the classic strains Golden teacher (Psilocybe cubensis) or Liberty caps (Psilocybe semilanceata). Since the small dose yields a sub-perceptual effect, there should be no explicit difference in which strain is used. If you do notice some effects, you took too much; try dialing back the dosage until you find your sweet spot.

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Buildroot features an infrastructure for packages that use kconfig fortheir configuration. This infrastructure provides the necessary logic toexpose the package’s menuconfig target as foo-menuconfig inBuildroot, and to handle the copying back and forth of the configurationfile in a correct way.

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The goal of the present study is to exemplify ways to valorize lignocellulosic food waste within the biorefinery concept. Rice straw was selected as the feedstock of focus, but the presented methodologies can be extended to other similar materials such as sugarcane bagasse, wheat straw, and corn stover. The research presents alternative and sustainable conceptual design strategies for the production of two major products of conventional refineries, that is, ethylene and jet fuel. The assessment provides a comprehensive approach that includes technical design, process modeling, and economic evaluation. Based on a plant treating 100 dry t/h of rice straw, 27/8 MWh of energy are required to generate a ton of ethylene and a 43/7% energy efficiency is realized for the aviation fuel case. The conduction of a typical discounted cash flow analysis resulted in minimum selling prices (MSP) of $1/34/kg for ethylene and $1/52/L for jet fuel. The conduction of a sensitivity analysis revealed that the processes are capital and feedstock intensive, while an increase to final products yield will favor the economic performance of the examined biorefineries.

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If the perl module links with a shared library that is provided by another (non-perl) package, this dependency is not added automatically. It has to be added manually to PERL_FOO_BAR_DEPENDENCIES.

LIBFOO_IGNORE_CVES is a space-separated list of CVEs that tells Buildroot CVE tracking tools which CVEs should be ignored for this package. This is typically used when the CVE is fixed by a patch in the package, or when the CVE for some reason does not affect the Buildroot package. A Makefile comment must always precede the addition of a CVE to this variable.

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You should still manually edit the result. In particular, thefollowing things should be checked.

LIBFOO_NEEDS_EXTERNAL_WAF can be set to YES or NO to tell Buildroot to use the bundled waf executable. If set to NO, the default, then Buildroot will use the waf executable provided in the package source tree; if set to YES, then Buildroot will download, install waf as a host tool and use it to build the package.

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Meson is an open source build system meant to be bothextremely fast, and, even more importantly, as user friendly as possible. Ituses Ninja as a companion tool to perform the actualbuild operations.

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In this case, we iterate over the values to build each target, and for each produced a binary that is the non-directory component of the target. For example if FOO_BUILD_TARGETS = cmd/docker cmd/dockerd the binaries produced are docker and dockerd.

Bzr for retrieving source code from a Bazaar repository. Used by default when LIBFOO_SITE begins with bzr://. The downloaded source code is cached as with the svn method.

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Buildroot offers a helper infrastructure to make it easy to write packages thatbuild and install Linux kernel modules. Some packages only contain a kernelmodule, other packages contain programs and libraries in addition to kernelmodules. Buildroot’s helper infrastructure supports either case.

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FOO_MODULE_SUBDIRS may be set to one or more sub-directories (relative to the package source top-directory) where the kernel module sources are. If empty or not set, the sources for the kernel module(s) are considered to be located at the top of the package source tree.

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As a policy, packages that merely provide Python modules should all benamed python-<something> in Buildroot. Other packages that use thePython build system, but are not Python modules, can freely choosetheir name (existing examples in Buildroot are scons andsupervisor).

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Foo-update-config, which copies the configuration back to the source configuration file. It is not possible to use this target when fragment files are set.

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LIBFOO_MAKE, to specify an alternate make command. This is typically useful when parallel make is enabled in the configuration (using BR2_JLEVEL) but that this feature should be disabled for the given package, for one reason or another.

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Psilocybin and its metabolites are not included in most standard drug screens. However, they are sometimes included in extended drug screens.

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Use a select type of dependency for dependencies on libraries. These dependencies are generally not obvious and it therefore make sense to have the kconfig system ensure that the dependencies are selected. For example, the libgtk2 package uses select BR2_PACKAGE_LIBGLIB2 to make sure this library is also enabled. The select keyword expresses the dependency with a backward semantic.

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This dependency download mechanism is not convenient when performing an offlinebuild, as Cargo will fail to fetch the dependencies. In that case, it is advisedto generate a tarball of the dependencies using the cargo vendor and add it toFOO_EXTRA_DOWNLOADS.

Local for a local source code directory. One should use this when LIBFOO_SITE specifies a local directory path containing the package source code. Buildroot copies the contents of the source directory into the package’s build directory. Note that for local packages, no patches are applied. If you need to still patch the source code, use LIBFOO_POST_RSYNC_HOOKS, see Section 18/22/1, “Using the POST_RSYNC hook”.

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To cover this requirement, Buildroot provides support for so-called users tables

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FOO_POST_RSYNC_HOOKS to run additional commands after the sources have been copied by Buildroot. This can for example be used to generate part of the manual with information extracted from the tree. As an example, Buildroot uses this hook to generate the tables in the appendices.

LIBFOO_CPE_ID_VERSION, specifies the version part of the CPE identifier. When not defined the default value is $(LIBFOO_VERSION).

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Of course the $(HOST_DIR), $(STAGING_DIR) and $(TARGET_DIR) variables to install the packages properly. Those variables point to the global host, staging and target directories, unless per-package directory support is used, in which case they point to the current package host, staging and target directories. In both cases, it doesn’t make any difference from the package point of view: it should simply use HOST_DIR, STAGING_DIR and TARGET_DIR. See Section 8/12, “Top-level parallel build” for more details about per-package directory support.

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LIBFOO_STRIP_COMPONENTS is the number of leading components (directories) that tar must strip from file names on extraction. The tarball for most packages has one leading component named "<pkg-name> -<pkg-version>", thus Buildroot passes -strip-components=1 to tar to remove it. For non-standard packages that don’t have this component, or that have more than one leading component to strip, set this variable with the value to be passed to tar.

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The kconfig-package infrastructure is based on the generic-packageinfrastructure. All variables supported by generic-package areavailable in kconfig-package as well. SeeSection 18/5.2, “generic-package reference” for more details.

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Objective: The objective of the study is to optimize the solubility of a drug or a drug-like molecule using Aspen Plus simulation platform. Aspirin (solute) was taken as the second case study. The following solvents were used in our dry (virtual) laboratory experiment: Water, acetone, ethanol, and ethylene-glycol-mono-propyl-ether (PROPGLYC). Methods: A simplified process flow sheet made of a single mixing tank where it has five feed streams, representing the solute, the water, and the set of three organic solvents, and one product stream where aspirin is either solubilized (liquid solution) or remains as solid crystal. Minimization of the molar Gibbs free energy of mixing, ∆G mix, was used as an objective function from an optimization point of view. The Non-random Two-liquid property method was used to analyze the solution properties. Results: Using the molar Gibbs free energy of mixing, ∆G mix, as a criterion of solution thermodynamic stability, it was found that the most stable solution is the quinary mixture made of 24/42% aspirin, 10/22% water, 21/08% acetone, 19/51% ethanol, and 24/77 mole % PROPGLYC. Conclusions: Exploiting Aspen Plus can be extended to handle the solubility of a new drug-like molecule once it is defined within its molecular editor with a little knowledge such as density and/or melting point.

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In the context ofBuildroot, the luarocks-package infrastructure only supports the builtinmode. LuaRocks packages that use the make or cmake build mechanismsshould instead be packaged using the generic-package and cmake-packageinfrastructures in Buildroot, respectively.

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LIBFOO_REDISTRIBUTE can be set to YES (default) or NO to indicate if the package source code is allowed to be redistributed. Set it to NO for non-opensource packages: Buildroot will not save the source code for this package when collecting the legal-info.

The dropout method is first utilized to reduce the overfitting of LSTM models to noisy data. Then, another approach termed co-teaching method is used to train LSTM models with additional noise-free data generated from simulations of the reactor first-principles model that employs several standard modeling assumptions not made in the Aspen model. Through open-loop and closed-loop simulations, we demonstrate the improvement of model prediction accuracy and of the open- and closed-loop performances under model predictive controllers using dropout and co-teaching LSTM neural network models compared to the LSTM model developed from the standard training process from the noisy data.

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The "baz" package is alsoadded to FOO_DEPENDENCIES. Note that the support for baz is explicitlydisabled at line 20, if the package is not selected.

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LIBFOO_SOURCE may contain the name of the tarball of the package, which Buildroot will use to download the tarball from LIBFOO_SITE. If HOST_LIBFOO_SOURCE is not specified, it defaults to LIBFOO_SOURCE.

Foo-update-defconfig, which copies the configuration back to the source configuration file. The configuration file will only list the options that differ from the default values. It is not possible to use this target when fragment files are set.

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However, in the mean time, you may want to store kernel or bootloaderconfiguration or patches specific to your target platform. To do so,create a directory board/<manufacturer> and a subdirectoryboard/<manufacturer>/<boardname>. You can then store your patchesand configurations in these directories, and reference them from the mainBuildroot configuration. Refer to Chapter 9, Project-specific customization for more details.

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Generate all the targets listed in the TARGETS variable. This variable is filled by all the individual components' Makefiles. Generating these targets will trigger the compilation of the userspace packages (libraries, programs), the kernel, the bootloader and the generation of the root filesystem images (see this here), depending on the configuration.

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Other host dsRNA PRRs, such as PKR and OAS, are also activated and operate to limit CoV replication (7⇓⇓⇓–11). CoVs encode multiple proteins that antagonize these innate immune responses, particularly the activation of the IFN response (9, 12⇓⇓⇓–16), ultimately leading to a dysregulated immune response and increased immunopathogenesis (17, 18). Understanding the mechanisms used by CoVs to delay IFN signaling may provide opportunities for the development of antivirals and live-attenuated vaccines to limit CoV infections.

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Hg for retrieving source code from a Mercurial repository. One must specify LIBFOO_SITE_METHOD=hg when LIBFOO_SITE contains a Mercurial repository URL. The downloaded source code is cached as with the svn method.

By packages with specific build systems we mean all the packageswhose build system is not one of the standard ones, such asautotools or CMake. This typically includes packages whose buildsystem is based on hand-written Makefiles or shell scripts.

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As mentioned above, Buildroot is basically a set of Makefiles thatdownload, configure, and compile software with the correct options. Italso includes patches for various software packages - mainly the onesinvolved in the cross-compilation toolchain (gcc, binutils anduClibc).

The cDNA was generated using cDNA primers specific to the negative-sense RNA, random hexamers for total RNA, or oligo-dT primers for positive-sense RNA. The qPCR was performed with either primer set 1 or primer set 2 for each polyU qPCR.

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This section covers how new packages (userspace libraries orapplications) can be integrated into Buildroot. It also shows howexisting packages are integrated, which is needed for fixing issues ortuning their configuration.

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Makefiles for generic packages (not using autotools or CMake): These are based on an infrastructure similar to the one used for autotools-based packages, but require a little more work from the developer. They specify what should be done for the configuration, compilation and installation of the package. This infrastructure must be used for all packages that do not use the autotools as their build system. In the future, other specialized infrastructures might be written for other build systems. We cover them through in a tutorial and a reference.

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Gcc _sync* built-ins used for atomic operations. They are available in variants operating on 1 byte, 2 bytes, 4 bytes and 8 bytes.

In Buildroot, a virtual package is a package whose functionalities areprovided by one or more packages, referred to as providers. The virtualpackage management is an extensible mechanism allowing the user to choosethe provider used in the rootfs.

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LIBFOO_BIN_ARCH_EXCLUDE is a space-separated list of paths (relative to the target directory) to ignore when checking that the package installs correctly cross-compiled binaries. You seldom need to set this variable, unless the package installs binary blobs outside the default locations, /lib/firmware, /usr/lib/firmware, /lib/modules, /usr/lib/modules, and /usr/share, which are automatically excluded.

One should use this when LIBFOO_SITE specifies a package tarball as a local filename. Useful for software that isn’t available publicly or in version control.

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On line 11, we tell Buildroot to not install the package to thetarget directory. This directory contains what will become the rootfilesystem running on the target. For purely static libraries, it isnot necessary to install them in the target directory because they willnot be used at runtime. By default, target installation is enabled; settingthis variable to NO is almost never needed. Also by default, packages areinstalled in this location using the make install command.

Since EndoU is conserved among CoVs, we sought to determine whether EndoU reduces the abundance and length of the PUN RNAs in the alpha-CoV porcine epidemic diarrhea virus (PEDV). Although the EndoU domains of MHV and PEDV exhibit only about 50% overall amino acid similarity, the catalytic histidines are 100% conserved (19). We showed that inactivation of EndoU in PEDV results in an increased type I and type III IFN response during infection (20). To determine whether EndoU limits the accumulation of PUN RNAs during PEDV infection, we infected cells with either wild-type or EndoUmut PEDV, isolated RNA, and evaluated the levels of PUN RNAs.

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LIBFOO_LICENSE defines the license (or licenses) under which the package is released. This name will appear in the manifest file produced by make legal-info. If the license appears in the SPDX License List, use the SPDX short identifier to make the manifest file uniform. Otherwise, describe the license in a precise and concise way, avoiding ambiguous names such as BSD which actually name a family of licenses.

The bool line, help line and other metadata information about theconfiguration option must be indented with one tab. The help textitself should be indented with one tab and two spaces, lines shouldbe wrapped to fit 72 columns, where tab counts for 8, so 62 charactersin the text itself. The help text must mention the upstream URL of theproject after an empty line.

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Cargo maintains a local cache of the registry index and of git checkouts of thecrates, whose location is given by $CARGO_HOME. As seen in the packageMakefile example at line 15, this environment variable is set to$(HOST_DIR)/share/cargo.

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IFNAR−/− BMDMs, C57BL/6 BMDMs, or AML12 cells were infected with wild-type or EndoUmut virus at a multiplicity of infection (MOI) of 1. At indicated times postinfection, RNA was isolated with RNeasy kit (74104; Qiagen). One microgram of RNA was mixed with dsRNA antibody (K1; Scicons) or mouse anti-Beta actin (A00702-40; Genscript), RNA binding buffer (50 mM Tris pH 8/0, 150 mM NaCl, 1% Nonidet P-40, and 20 U/mL Ribolock [EO0381; Thermofisher]). After overnight incubation, protein G beads (LSKMAGG02; Millipore) were added and incubated rotating for 4 h at 4 °C. Protein G beads were precipitated with magnets, then washed with cold binding buffer. RNA was purified off beads with RNeasy Kit and reverse-transcribed into cDNA with RT2 First Strand kit (330411; Qiagen). The qPCR was performed as described below.

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LIBFOO_FLAT_STACKSIZE defines the stack size of an application built into the FLAT binary format. The application stack size on the NOMMU architecture processors can’t be enlarged at run time. The default stack size for the FLAT binary format is only 4k bytes. If the application consumes more stack, append the required number here.

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LIBFOO_DL_OPTS is a space-separated list of additional options to pass to the downloader. Useful for retrieving documents with server-side checking for user logins and passwords, or to use a proxy. All download methods valid for LIBFOO_SITE_METHOD are supported; valid options depend on the download method (consult the man page for the respective download utilities).

This is especially important if thedifferent versions have different licensing terms, but they are storedin the same file. Otherwise, the hash file should stay in the package’sdirectory.

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If any of those variables is defined, then the generic packageinfrastructure assumes the package provides valid CPE information. Inthis case, the generic package infrastructure will defineLIBFOO_CPE_ID.

LIBFOO_SITE_METHOD determines the method used to fetch or copy the package source code. In many cases, Buildroot guesses the method from the contents of LIBFOO_SITE and setting LIBFOO_SITE_METHOD is unnecessary. When HOST_LIBFOO_SITE_METHOD is not specified, it defaults to the value of LIBFOO_SITE_METHOD.

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Cells carry sensors that are primed to detect invading viruses. To avoid being recognized, coronaviruses express factors that interfere with host immune sensing pathways. Previous studies revealed that a coronavirus endoribonuclease (EndoU) delays activation of the host sensor system, but the mechanism was not known. Here, we report that EndoU cleaves a viral polyuridine sequence that would otherwise activate host immune sensors. This information may be used in developing inhibitors that target EndoU activity and prevent diseases caused by coronaviruses.

Some packages need a Linux kernel to be built by buildroot. These aretypically kernel modules or firmware.

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LIBFOO_INSTALL_TARGET_CMDS lists the actions to be performed to install the package to the target directory, when the package is a target package. The package must install its files to the directory given by $(TARGET_DIR). Only the files required for execution of the package have to be installed. Header files, static libraries and documentation will be removed again when the target filesystem is finalized.

The main macro of the Go package infrastructure isgolang-package. It is similar to the generic-package macro.